Resume
Work Experience
Jan. 2022 - Present
Post doctoral researcher at LMU Munich
I am currently a postdoc at BMC Munich, where I support chromatin biologists in advancing the understanding of chromatin structure and function through data analysis. My work involves analyzing bulk and single-cell RNA-seq data and communicating the results via easy-to-understand charts, plots, and R Markdown reports to collaborators. To ensure reproducibility, I encode the analyses into Snakemake pipelines. Additionally, I independently conduct research in prompt engineering, knowledge graphs, and biomedical information retrieval, aiming to enhance information extraction from research papers. I also develop full-stack AI applications using HTML, CSS, JavaScript, R, and Python.
Sep. 2015 - Dec. 2021
wissenschaftlicher Mitarbeiter at LMU Munich
During my PhD, I worked on multiple projects involving the analysis, integration, and visualization of high-throughput proteomics data from imaging and liquid chromatography-based mass spectrometry experiments. This work led to the development of command-line software pipelines in R. To facilitate ease of use for non-technical users, some of these pipelines were integrated into R Shiny apps and an Electron JS application.
Apr. 2012 - Dec. 2012
Senior software engineer at iCelero Banglore
I joined iCelero, a U.S.-based software company in Bangalore, as a Senior Software Engineer, where I worked on cutting-edge technologies such as Selenium WebDriver, Python, Java, and Autoit. At iCelero, I contributed to the development of a software pipeline to automate software testing.
Aug. 2009 - Mar. 2012
Research assistant at JNCASR
Following the successful completion of my BE project, I joined Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) as a research assistant. At JNCASR, I contributed to a numerical computation project where I developed a C program to diagonalize large sparse matrices, a crucial tool for solving quantum chemistry problems, particularly those involving large-scale configuration interaction calculations of electronic wave functions. I also participated in a study investigating the low-energy properties of spinless fermions in two coupled chains using the density matrix renormalization group method. In collaboration with biologists at the institute, I developed a C program to predict the functions of hypothetical proteins using structural features from the Protein Data Bank (PDB). Additionally, I assisted in debugging the Fortran implementation of the ZINDO (Zerner's Intermediate Neglect of Differential Overlap) software to address memory leak issues.
Feb. 2009 - Jun. 2009
Intern at JNCASR
During my internship at JNCASR, I worked on my final year Bachelor's degree thesis, which involved developing a C program for the iterative calculation of the lowest eigenvalues and eigenvectors of large real-symmetric matrices.
Education
Sep. 2015 - Dec. 2021
Ludwig Maximilian University of Munich | PhD in Computational Proteomics
In the past decade, proteomics data has accumulated rapidly, posing numerous computational challenges. During my Ph.D., I tackled three such challenges that demanded significant computational effort.
Jan. 2013 - May 2015
King AbdulAziz University | MSc. in Computer Engineering
During MS, I studied computational biology, algorithms, and machine learning. My research was targeted to extract meaningful information from complex systems like human PDZ domains-protein interaction network.
Sep. 2005 - Aug. 2009
Visvesvaraya Technological University | BE in Computer Engineering
During my bachelor's degree, I gained a solid foundation in both theoretical and practical aspects of core computer science subjects, including discrete mathematics, data structures, and algorithms. In my final year, I interned at the JNCASR. For my BE project, I developed a C program for the iterative calculation of the lowest eigenvalues and eigenvectors of large real-symmetric matrices.
Publications
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Optimizing biomedical information retrieval with a keyword frequency-driven prompt
enhancement strategy.
Aftab W, Apostolou Z, Bouazoune K, Straub T. BMC Bioinformatics. 2024 Aug 27;25(1):281. doi: 10.1186/s12859-024-05902-7. -
Improving SWATH-MS analysis by deep-learning.
Sun B, Smialowski P, Aftab W, Schmidt A, Forne I, Straub T, Imhof A. Proteomics. 2023 May;23(9):e2200179. doi: 10.1002/pmic.202200179. Epub 2023 Jan 3. -
Discovery of Native Protein Complexes by Liquid Chromatography Followed by Quantitative
Mass
Spectrometry.
Aftab W, Imhof A. Adv Exp Med Biol. 2021;1336:105-128. doi: 10.1007/978-3-030-77252-9_6.
PMID: 34628629 -
ImShot: An Open-Source Software for Probabilistic Identification of Proteins In Situ and
Visualization of Proteomics Data.
Aftab W, Lahiri S, Imhof A. Mol Cell Proteomics. 2022 Jun;21(6):100242. doi: 10.1016/j.mcpro.2022.100242. Epub 2022 May 13. -
MALDI-IMS combined with shotgun proteomics identify and localize new factors in male
infertility.
Lahiri S, Aftab W, Walenta L, Strauss L, Poutanen M, Mayerhofer A, Imhof A. Life Sci Alliance. 2021 Jan 6;4(3):e202000672. doi: 10.26508/lsa.202000672. Print 2021 Mar. -
Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune
antagonists and remaining vulnerabilities.
Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Müller JA, Srinivasachar Badarinarayan S, Stürzel CM, Forne I, Stenger S, Conzelmann KK, Münch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ. Cell Rep. 2021 May 18;35(7):109126. doi: 10.1016/j.celrep.2021.109126. Epub 2021 Apr 27. -
The Integrity of the HMR complex is necessary for centromeric binding and reproductive
isolation in Drosophila.
Lukacs A, Thomae AW, Krueger P, Schauer T, Venkatasubramani AV, Kochanova NY, Aftab W, Choudhury R, Forne I, Imhof A. PLoS Genet. 2021 Aug 23;17(8):e1009744. doi: 10.1371/journal.pgen.1009744. eCollection 2021 Aug. -
Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS
Biogenesis.
Singh AP, Salvatori R, Aftab W, Kohler A, Carlström A, Forne I, Imhof A, Ott M. Mol Cell. 2020 Sep 17;79(6):1051-1065.e10. doi: 10.1016/j.molcel.2020.07.024. Epub 2020 Sep 1. -
Mapping protein networks in yeast mitochondria using proximity-dependent biotin
identification coupled to proteomics.
Salvatori R, Aftab W, Forne I, Imhof A, Ott M, Singh AP. STAR Protoc. 2020 Dec 15;1(3):100219. doi: 10.1016/j.xpro.2020.100219. eCollection 2020 Dec 18. -
Molecular Wiring of a Mitochondrial Translational Feedback Loop.
Salvatori R, Kehrein K, Singh AP, Aftab W, Möller-Hergt BV, Forne I, Imhof A, Ott M. Mol Cell. 2020 Feb 20;77(4):887-900.e5. doi: 10.1016/j.molcel.2019.11.019. Epub 2019 Dec 26.
PMID: 31883951 Free article. -
Msp1 cooperates with the proteasome for extraction of arrested mitochondrial import
intermediates.
Basch M, Wagner M, Rolland S, Carbonell A, Zeng R, Khosravi S, Schmidt A, Aftab W, Imhof A, Wagener J, Conradt B, Wagener N. Mol Biol Cell. 2020 Apr 1;31(8):753-767. doi: 10.1091/mbc.E19-06-0329. Epub 2020 Feb 12.
PMID: 32049577 Free PMC article. -
A Novel Kernel for RBF Based Neural Networks.
Wasim Aftab, Muhammad Moinuddin, Muhammad Shafique Shaikh. Abstract and Applied Analysis. Volume 2014, Issue 1. 10.1155/2014/176253 First published: 19 June 2014. -
On Classification of PDZ Domains: A Computational Study.
Wasim Aftab, Adnan Memic, Dumitru Baleanu. Mathematical Problems in Engineering. Volume 2013, Issue 1. 10.1155/2013/419485 First published: 20 October 2013.